Leucine degradation in sheep

Initial studies on leucine metabolism in the rumen of sheep.

1. [3H]leucine infused directly into the rumen of sheep labelled microbial protein and, when compared with the specific activity of the rumen free-leucine pool, indicated that 50% of the bacterial protein leucine originated from the rumen free-leucine pool. 2. The lower limit for bacterial protein turnover in the rumen was 0.37/d when calculated as the difference between the specific rate of di...

Degradation of Duckweed Protein in the Rumen of Sheep

Degradation of plant N in the rumen results in the formation of ammonia-N and NAN. Rumen bacteria utilise these breakdown products to synthesise their cell proteins. This study was carried out to investigate the in vivo degradation of N-labelled duckweed in the rumen and the utilisation of the degradation products by rumen bacteria. We found that duckweed protein was relatively resistant to rum...

Effect of protozoa on rumen protein degradation in sheep.

We have studied the contribution of ciliate protozoa to the degradation of dietary protein, utilizing 5 defaunated and 6 faunated sheep. Rumen samples from these animals were used as inocular for in vitro determination of the degradability of different protein sources: lupine grain, peanut and soybean cake, and fish meal. We also applied the in sacco method to measure the digestibility of soybe...

Does leucine, leucyl-tRNA, or some metabolite of leucine regulate protein synthesis and degradation in skeletal and cardiac muscle?

Comparison of the [H5]Phenylalanine Model with the [1-C]Leucine Method to Determine Whole Body Protein Synthesis and Degradation in Sheep Fed at Two Levels

The [H5]phenylalanine model was compared with the [1-C]leucine method to determine whole body protein synthesis (WBPS) and degradation (WBPD) in sheep fed at two levels. The animals were fed either 103 (M-diet) or 151 (H-diet) kcal ME/kg/day once daily in a crossover design for 21 days each. The isotope dilutions were simultaneously conducted as a primedcontinuous infusion of [H5]phenylalanine,...